3BO2
A relaxed active site following exon ligation by a group I intron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 108.960, 108.960, 249.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.310 |
| R-factor | 0.292 |
| Rwork | 0.291 |
| R-free | 0.32100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 22911 | |
| Completeness [%] | 99.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 298 | MPD, Sodium Cacodylate, Magnesium Acetate, Potassium Acetate, Cobalt Hexamine, Potassium Vanadate, pH 6.80, vapor diffusion, hanging drop, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 1 | Potassium Vanadate | ||
| 2 | 1 | 2 | MPD | ||
| 3 | 1 | 1 | Sodium Cacodylate | ||
| 4 | 1 | 2 | Sodium Cacodylate | ||
| 5 | 1 | 1 | Magnesium Acetate | ||
| 6 | 1 | 2 | Magnesium Acetate | ||
| 7 | 1 | 1 | Potassium Acetate | ||
| 8 | 1 | 2 | Potassium Acetate | ||
| 9 | 1 | 1 | Cobalt Hexamine |
