3BNW
Crystal structure of riboflavin kinase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.695, 65.604, 62.690 |
| Unit cell angles | 90.00, 103.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.21616 |
| Rwork | 0.213 |
| R-free | 0.28857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Hybrid model generated by Rosetta |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.600 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.064 |
| Number of reflections | 10719 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 88.0 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 290 | 20% PEG 3350, 0.28M KF, 0.1M HEPES, 5mM DTT, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
