3BNP
Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site (A1555G Mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.350, 66.350, 57.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.590 - 2.700 |
| Rwork | 0.231 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.590 | 40.590 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.065 | 0.046 | 0.362 |
| Total number of observations | 3856 | 4343 | |
| Number of reflections | 4215 | ||
| <I/σ(I)> | 22 | 48.1 | 3.9 |
| Completeness [%] | 99.5 | 94.1 | 100 |
| Redundancy | 9.94 | 8.85 | 10.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, KCl, glycerol, paromomycin, 2-methyl-2,4-pentanediol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Sodium cacodylate | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | KCl |
