3BNE
Lipoxygenase-1 (Soybean) I553A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-10 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.115, 92.951, 49.416 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 29.720 - 1.400 |
| R-factor | 0.16655 |
| Rwork | 0.166 |
| R-free | 0.18603 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.400 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.574 |
| Number of reflections | 164852 | |
| <I/σ(I)> | 25.9 | 1.9 |
| Completeness [%] | 99.5 | 94.6 |
| Redundancy | 4.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 3350, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
