3BNB
Lipoxygenase-1 (Soybean) I553L Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-10 |
| Detector | SBC-3 |
| Wavelength(s) | 1.00686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.421, 92.726, 49.416 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 29.370 - 1.450 |
| R-factor | 0.16682 |
| Rwork | 0.166 |
| R-free | 0.18684 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.100 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.049 | 0.531 |
| Number of reflections | 144404 | |
| <I/σ(I)> | 26.3 | 2.1 |
| Completeness [%] | 95.9 | 85.2 |
| Redundancy | 4.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG3350, SODIUM ACETATE, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
