3BN8

Crystal structure of a putative sterol carrier protein type 2 (af1534) from archaeoglobus fulgidus dsm 4304 at 2.11 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91840, 0.97953, 0.97939
Spacegroup name	C 2 2 21
Unit cell lengths	56.250, 105.210, 85.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.571 - 2.110
R-factor	0.198
Rwork	0.196
R-free	0.24800
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.571	28.571	2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.039	0.024	0.231
Number of reflections	14873	2663	2622
<I/σ(I)>	12.16	27.5	3.2
Completeness [%]	97.6	92.8	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	NANODROP, 20.0% PEG 1000, 0.1M Tris-HCl pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K