3BN7

CRYSTAL STRUCTURE OF A DIMERIC FERREDOXIN-LIKE PROTEIN (CC_2267) FROM CAULOBACTER CRESCENTUS CB15 AT 1.64 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97954, 0.97916
Spacegroup name	P 65 2 2
Unit cell lengths	47.921, 47.921, 167.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.463 - 1.640
R-factor	0.2
Rwork	0.199
R-free	0.23300
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.713
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.463	29.463	1.680
High resolution limit [Å]	1.640	7.330	1.640
Rmerge	0.073	0.039	1.096
Total number of observations		1135	7377
Number of reflections	14906
<I/σ(I)>	5.7	14.8	0.7
Completeness [%]	100.0	98.3	100
Redundancy	6.8	4.8	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	NANODROP, 40.0% PEG 400, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K