3BN4
Carboxysome Subunit, CcmK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.900, 69.770, 78.010 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 60.300 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.366 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.128 | 0.102 | 0.515 |
| Number of reflections | 43130 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 98.8 | 97.6 | 97.8 |
| Redundancy | 5.3 | 5.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MES, 15% MPD, 0.1M lithium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
