Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-11-30 |
| Detector | SIEMENS |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 91.440, 91.440, 107.750 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| Rwork | 0.242 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tfg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.500 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.041 | 0.123 |
| Number of reflections | 4647 | |
| <I/σ(I)> | 24.2 | 5.4 |
| Completeness [%] | 94.6 | 82.2 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.4 | 20 * | CRYSTALLIZATION CONDITIONS: PROTEIN WAS CRYSTALLIZED FROM 100 MM LITHIUM SULFATE, 12% TERT-BUTANOL, 50 MM CITRATE, PH 5.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | acetate | 20 (mM) | |
| 3 | 1 | reservoir | citrate | 50 (mM) | |
| 4 | 1 | reservoir | lithium sulfate | 100 (mM) | |
| 5 | 1 | reservoir | tertbutanol | 12 (%(v/v)) |
