3BMB

Crystal structure of a new RNA polymerase interacting protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Detector technology	CCD
Collection date	2005-02-05
Detector	MAR CCD 165 mm
Wavelength(s)	1.74326
Spacegroup name	C 2 2 21
Unit cell lengths	68.500, 133.400, 66.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 1.910
Rwork	0.213
R-free	0.23700
RMSD bond length	0.006
RMSD bond angle	1.402
Data reduction software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.980
High resolution limit [Å]	1.910	1.910
Number of reflections	22457
<I/σ(I)>	53.3	13.7
Completeness [%]	98.8	97.5
Redundancy	17.2	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	50mM Mes, 10mM MgCl2, 1.5M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	50mM Mes, 10mM MgCl2, 1.5M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K