3BLZ

Crystal structure of a ntf2-like protein of unknown function (sbal_0622) from shewanella baltica os155 at 1.75 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9796
Spacegroup name	P 1
Unit cell lengths	57.085, 58.121, 140.462
Unit cell angles	80.93, 78.43, 60.60

Refinement procedure

Resolution	29.348 - 1.750
R-factor	0.154
Rwork	0.152
R-free	0.19400
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.446
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.348	29.348	1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.042	0.019	0.456
Total number of observations		3296	8054
Number of reflections	126809
<I/σ(I)>	11.9	26	1.5
Completeness [%]	81.7	94.8	37.8
Redundancy	1.9	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	NANODROP, 20.0% 2-propanol, 20.0% PEG 4000, 0.1M Citric acid pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K