3BLD
tRNA guanine transglycosylase V233G mutant preQ1 complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97803 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.655, 64.878, 70.328 |
Unit cell angles | 90.00, 95.55, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.190 |
Rwork | 0.151 |
R-free | 0.18540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bl3 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.028 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 8.000 |
High resolution limit [Å] | 1.190 |
Number of reflections | 116963 |
<I/σ(I)> | 21 |
Completeness [%] | 91.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris/HCl, 1mM DTT, 5% PEG 8000, 10% DMSO, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |