3BL6
Crystal structure of Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in complex with formycin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.250, 81.670, 45.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.800 - 1.700 |
Rwork | 0.181 |
R-free | 0.18700 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.800 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.028 | 0.110 |
Number of reflections | 24529 | |
<I/σ(I)> | 19.9 | 5.8 |
Completeness [%] | 93.1 | 88.1 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapour diffusion, hanging drop | 7.5 | 298 | PEG 8000, Potassium phosphate monobasic, pH 7.5, vapour diffusion, hanging drop, temperature 298K |