3BL2
Crystal Structure of M11, the BCL-2 Homolog of Murine Gamma-herpesvirus 68, Complexed with Mouse Beclin1 (residues 106-124)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2006-09-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.863, 53.601, 73.586 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.204 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2abo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.063 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.064 | 0.044 | 0.162 |
| Number of reflections | 13513 | ||
| <I/σ(I)> | 14.4 | ||
| Completeness [%] | 94.0 | 99.1 | 80.5 |
| Redundancy | 4.9 | 6.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 25% PEG 3350, 0.2M MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
