3BKW

Crystal structure of S-adenosylmethionine dependent methyltransferase (NP_104914.1) from Mesorhizobium loti at 1.60 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97901
Spacegroup name	P 21 21 21
Unit cell lengths	58.330, 58.480, 142.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.235 - 1.600
R-factor	0.173
Rwork	0.171
R-free	0.20000
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.235	29.235	1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.072	0.036	0.725
Number of reflections	64958	12372	12870
<I/σ(I)>	10.55	30.6	1.4
Completeness [%]	99.8	99.2	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	277	NANODROP, 0.2M Mg Acetate, 20.0% PEG 3350, No Buffer pH 7.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K