3BKV

X-ray structure of the bacteriophage phiKZ lytic transglycosylase, gp144, in complex with chitotetraose, (NAG)4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-05-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	62.322, 62.322, 182.643
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.390 - 2.600
R-factor	0.227
Rwork	0.227
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Structure of the phiKZ gp144 (PDB code 3BKH)
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS (1.2)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.390	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	12279
<I/σ(I)>	45.6	14
Completeness [%]	99.7	99
Redundancy	8.6	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.2% (v/v) polyethylene glycol 400, 60 mM HEPES sodium, pH 7.5. Presence of DTT (5mM) in the protein solution was important for crystallization, VAPOR DIFFUSION, HANGING DROP, temperature 293K