Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.905 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.523, 151.520, 116.715 |
| Unit cell angles | 90.00, 128.08, 90.00 |
Refinement procedure
| Resolution | 29.640 - 2.720 |
| R-factor | 0.18144 |
| Rwork | 0.179 |
| R-free | 0.22828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sof |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.715 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.760 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.110 | 0.528 |
| Number of reflections | 65786 | |
| <I/σ(I)> | 10 | 2.3 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 100mM HEPES pH 7.5, 10% (v/v) 2-propanol, 14% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
