3BKF

Zinc-bound C-terminal Domain of NikR

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.2829, 1.0
Spacegroup name	P 62 2 2
Unit cell lengths	46.220, 46.220, 124.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.900
R-factor	0.251
Rwork	0.250
R-free	0.27300
Starting model (for MR)	MONOMER OF PDB 1Q5Y
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	6755
<I/σ(I)>	28.1	3.4
Completeness [%]	99.5
Redundancy	9.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M Bis-Tris, 0.2M Ammonium Sulfate, 25% w/v PEG 3350, pH 6.0, vapor diffusion, hanging drop, temperature 298K