3BKC
Crystal structure of anti-amyloid beta FAB WO2 (P21, FormB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.404, 111.164, 53.090 |
| Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
| Resolution | 51.570 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.23600 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.680 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.571 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.164 |
| Number of reflections | 33358 | |
| <I/σ(I)> | 14.4 | 4.8 |
| Completeness [%] | 93.2 | 68.1 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 25% (v/v) PEG 550 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
