3BJV
The Crystal Structure of a putative PTS IIA(PtxA) from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2007-12-02 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 40.754, 40.754, 169.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.280 - 2.400 |
| R-factor | 0.166 |
| Rwork | 0.154 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oqt |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.826 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.650 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.130 |
| Number of reflections | 6026 | |
| <I/σ(I)> | 10.08 | 0.1297 |
| Completeness [%] | 97.1 | 85.1 |
| Redundancy | 9.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.9 | 289 | 0.1M Tris-Hcl, 1.9-2.1M (NH4)2SO4, pH 7.9, Vapor diffusion, temperature 289K |
