3BJP
Urate oxidase cyanide uric acid ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.936, 94.994, 104.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.2106 |
| Rwork | 0.207 |
| R-free | 0.24290 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2icq |
| RMSD bond length | 0.030 |
| RMSD bond angle | 0.075 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.226 |
| Number of reflections | 32677 | |
| <I/σ(I)> | 21.4 | 4.1 |
| Completeness [%] | 96.2 | 98.4 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 291 | 8.5MG/ML URATE OXIDASE, 0.2M SODIUM CYANIDE, 50MM TRIS BUFFER, 0.5MG/ML URIC ACID, 10% PEG 8000, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
