3BJH
Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.
Replaces: 1R5RExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.386, 86.289, 50.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.17495 |
Rwork | 0.171 |
R-free | 0.20645 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.355 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.800 | 1.680 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.062 | 0.305 |
Number of reflections | 22274 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 5.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.5M ammonium sulfate, 0.15M sodium citrate, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |