3BJD
Crystal structure of putative 3-oxoacyl-(acyl-carrier-protein) synthase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.566, 156.904, 136.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 1.850 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.20330 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.363 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.079 | 0.552 |
| Number of reflections | 87637 | |
| <I/σ(I)> | 12.9 | 2.04 |
| Completeness [%] | 98.7 | 84.8 |
| Redundancy | 13.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 0.2 M Magnesium chloride, 30% PEG 4000, 0.1 M Sodium cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
