3BJ3
met-Perch hemoglobin at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.730, 84.897, 121.475 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.500 - 2.100 |
| R-factor | 0.20666 |
| Rwork | 0.204 |
| R-free | 0.25205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ouu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 32953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1 M Tris at pH 8.0 and 16.5% polyethylene glycol 4K, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
