3BIW
Crystal structure of the Neuroligin-1/Neurexin-1beta synaptic adhesion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2007-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.830, 148.796, 123.602 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 45.900 - 3.500 |
| Rwork | 0.246 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.530 |
| High resolution limit [Å] | 3.410 | 7.340 | 3.410 |
| Rmerge | 0.093 | 0.048 | 0.688 |
| Number of reflections | 53484 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 92.5 | 100 | 71.2 |
| Redundancy | 3 | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 8% PEG6000, 0.1 M MgCl2, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
