3BIT
Crystal structure of yeast Spt16 N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.683, 144.635, 88.513 |
| Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
| Resolution | 39.880 - 1.900 |
| R-factor | 0.17 |
| Rwork | 0.167 |
| R-free | 0.22400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.377 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.455 |
| Number of reflections | 73962 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 94.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 278 | 35% PEG 300, 200mM NaCl, 100mM Sodium Acetate, pH 4.5, vapor diffusion, temperature 278K |
