3BIH
Crystal structure of fructose-1,6-bisphosphatase from E.coli GlpX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 91.381, 91.381, 86.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.100 |
| R-factor | 0.19104 |
| Rwork | 0.188 |
| R-free | 0.24985 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ni9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.643 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 21967 | |
| <I/σ(I)> | 30.5 | 3 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 9.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 28% PEG 400, 0.2M CaCl2, 0.1M Na Hepes pH 7.5, 10mM Fructose-6-phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
