3BHP
Crystal structure of UPF0291 protein ynzC from Bacillus subtilis at resolution 2.0 A. Northeast Structural Genomics Consortium target SR384
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.279, 38.460, 86.785 |
| Unit cell angles | 90.00, 107.95, 90.00 |
Refinement procedure
| Resolution | 19.550 - 2.010 |
| R-factor | 0.2289 |
| Rwork | 0.229 |
| R-free | 0.26230 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.822 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.123 |
| Number of reflections | 26190 | |
| <I/σ(I)> | 25.4 | 10.7 |
| Completeness [%] | 98.9 | 99.2 |
| Redundancy | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1M KH2PO4, 0.1M Na Citrate, 40% PEG 1000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
