3BHO

Crystal Structure of the 25kDa Subunit of Human Cleavage factor Im with Ap4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-12-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	79.955, 79.955, 72.186
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.800
Rwork	0.202
R-free	0.22500
Structure solution method	Isomorphous Replacement
Starting model (for MR)	3bap
RMSD bond length	0.005
RMSD bond angle	1.269
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.138	0.260
Number of reflections	46800
<I/σ(I)>	66	4.4
Completeness [%]	97.9
Redundancy	21.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	25% PEG 3350, 0.025M Magnesium chloride, 0.1M Tris-HCL pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	25% PEG 3350, 0.025M Magnesium chloride, 0.1M Tris-HCL pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K