3BH7

Crystal structure of the RP2-Arl3 complex bound to GDP-AlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	73.698, 77.943, 97.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 1.900
R-factor	0.23336
Rwork	0.232
R-free	0.26194
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ksg 2bx6
RMSD bond length	0.007
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	44554
<I/σ(I)>	20.03	5.3
Completeness [%]	98.7	98.5
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 3350, 0.4M potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K