3BH1
Crystal structure of protein DIP2346 from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-02-26 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.453, 138.936, 98.266 |
| Unit cell angles | 90.00, 116.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.21188 |
| Rwork | 0.211 |
| R-free | 0.27134 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 78192 | |
| <I/σ(I)> | 4.4 | 1.1 |
| Completeness [%] | 97.6 | 81.1 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 100mM Bis-Tris pH 6.0, 30% PEG 3350, 200mM Ammonium acetate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
