3BGY
Triclinic structure of Mimivirus Capping Enzyme Triphosphatase at 1.65 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2007-09-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.601, 1.21 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.640, 48.460, 69.700 |
| Unit cell angles | 74.62, 86.48, 76.36 |
Refinement procedure
| Resolution | 33.830 - 1.650 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.193 | |
| Number of reflections | 51783 | |
| <I/σ(I)> | 3.65 | |
| Completeness [%] | 85.1 | 66.6 |
| Redundancy | 57.88 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.0, VAPOR DIFFUSION, HANGING DROP |
