3BGW
The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.634, 184.411, 184.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.810 - 3.910 |
| R-factor | 0.33858 |
| Rwork | 0.338 |
| R-free | 0.34889 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.084 | 0.051 |
| Number of reflections | 35317 | |
| <I/σ(I)> | 23 | 2.5 |
| Completeness [%] | 98.0 | 92.1 |
| Redundancy | 6.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Hepes (pH 7.5), 1 1.25 M MgAc, and 0.02 0.04% n-octylglucoside, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
