3BGU

Crystal structure of a dimeric ferredoxin-like protein of unknown function (tfu_0763) from thermobifida fusca yx at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537, 0.9798, 0.9797
Spacegroup name	P 1 2 1
Unit cell lengths	44.070, 35.270, 76.630
Unit cell angles	90.00, 104.09, 90.00

Refinement procedure

Resolution	27.206 - 1.500
R-factor	0.199
Rwork	0.197
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.771
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.206	27.206	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.044	0.021	0.397
Number of reflections	35092	6954	4106
<I/σ(I)>	11.24	29.3	1.6
Completeness [%]	91.4	96.4	61.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	NANODROP, 50.0% Ethylene glycol, 5.0% PEG 1000, 0.1M Sodium acetate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K