3BG2
Crystal structure of deoxyguanosinetriphosphate triphosphohydrolase from Flavobacterium sp. MED217
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 |
| Unit cell lengths | 200.403, 200.403, 86.239 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.930 - 1.950 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.068 |
| Number of reflections | 92949 |
| Completeness [%] | 98.7 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% PEG 3000, 0.1M Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
