3BFQ
Crystal structure of truncated FimG (FimGt) in complex with the donor strand peptide of FimF (DSF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.050, 24.500, 54.150 |
| Unit cell angles | 90.00, 113.77, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.340 |
| R-factor | 0.132 |
| R-free | 0.17900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.064 | 0.234 |
| Number of reflections | 25442 | |
| <I/σ(I)> | 13.4 | 3.17 |
| Completeness [%] | 96.3 | 84.5 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM Tris/HCl, pH 8.0, 80 mM NaCl, 27.5% PEG 1500, 20 mM cobalt chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
