3BFK
Crystal structure of Plasmodium falciparum Rab11a in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.490, 48.371, 119.236 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.800 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oiv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.342 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 3.810 | 1.770 |
| Rmerge | 0.183 | 0.148 | 0.801 |
| Number of reflections | 91227 | ||
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 97.9 | 99.2 | 90.5 |
| Redundancy | 3.3 | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.9 | 293 | 26% PEG 4000, 100mM Na Citrate, 200mM NH4 Acetate, pH 5.9, VAPOR DIFFUSION, temperature 293K |
