3BF0
Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.171, 153.485, 100.659 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.550 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.765 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.122 | 0.324 |
| Number of reflections | 86579 | |
| <I/σ(I)> | 16 | 3.2 |
| Completeness [%] | 96.2 | 80.8 |
| Redundancy | 7.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG3350, pH 7.5, vapor diffusion, hanging drop, temperature 291K |
