3BEW
10mer Crystal Structure of chicken MHC class I haplotype B21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97650 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.393, 88.711, 100.230 |
| Unit cell angles | 90.00, 80.01, 90.00 |
Refinement procedure
| Resolution | 29.150 - 2.600 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bev |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.336 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.097 | 0.323 |
| Number of reflections | 39205 | |
| <I/σ(I)> | 13.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 27% PEG 3350, 0.1M HEPES pH 7.0, 0.1M MgCl2, vapor diffusion, sitting drop, temperature 293K |
