3BDW
Human CD94/NKG2A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-26 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.651, 34.730, 152.896 |
Unit cell angles | 90.00, 89.72, 90.00 |
Refinement procedure
Resolution | 30.590 - 2.500 |
R-factor | 0.236 |
Rwork | 0.234 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b6e |
RMSD bond length | 0.009 |
RMSD bond angle | 1.234 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.500 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 16457 | |
<I/σ(I)> | 10.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 298 | 1.0M tri-sodium citrate, 0.1M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |