3BDR

Crystal structure of fatty acid-binding protein-like Ycf58 from Thermosynecoccus elongatus. Northeast Structural Genomics Consortium target TeR13.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97900
Spacegroup name	P 41 21 2
Unit cell lengths	75.014, 75.014, 83.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 2.800
R-factor	0.236
Rwork	0.236
R-free	0.26200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.081	0.691
Number of reflections	11252
<I/σ(I)>	28.2	4.6
Completeness [%]	99.9	100
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	293	18% PEG 20000, 0.1M NH4H2PO4, 0.1M CAPS, 20% Glycerol, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K