3BDP

DNA POLYMERASE I/DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1997-08-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	87.063, 93.547, 106.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.25
Rwork	0.250
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bdp
RMSD bond length	0.012
RMSD bond angle	25.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.061 *	0.187 *
Total number of observations	326641 *
Number of reflections	63997
Completeness [%]	92.8	75.4
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8	18 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	55 (%(v/v)sat)
3	1	reservoir	MES	100 (mM)
4	1	reservoir	MPD	2.5-7.0 (%(v/v))
5	1	reservoir	sodium azide	0.02 (%(w/v))