3BDN

Crystal Structure of the Lambda Repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	74.431, 161.099, 135.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.610 - 3.909
R-factor	0.29597
Rwork	0.292
R-free	0.37413
Starting model (for MR)	1lmb 1f39
RMSD bond length	0.020
RMSD bond angle	1.773
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.610	4.140
High resolution limit [Å]	3.909	3.909
Rmerge	0.048	0.176
Number of reflections	7238
<I/σ(I)>	21.2	8
Completeness [%]	99.0	100
Redundancy	10	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	14% PEG 4000, 0.1 M Sodium Acetate pH 4.6, 0.2 M Ammonium Sulfate, 0.05 M Magnesium Chloride, 20% Glycerol, 1 mM Spermine, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
10	1	2	MgCl2
11	1	2	Glycerol
12	1	2	spermine
2	1	1	Sodium Acetate
3	1	1	(NH4)2SO4
4	1	1	MgCl2
5	1	1	Glycerol
6	1	1	spermine
7	1	2	PEG 4000
8	1	2	Sodium Acetate
9	1	2	(NH4)2SO4