3BDM
yeast 20S proteasome:glidobactin A-complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-06-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.770, 302.790, 143.210 |
| Unit cell angles | 90.00, 112.24, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Enty 1RYP |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.125 | 0.373 |
| Number of reflections | 368665 | |
| <I/σ(I)> | 13.3 | 3 |
| Completeness [%] | 96.0 | 85.8 |
| Redundancy | 2.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 294 | 100mM Mes, 14% MPD, 30mM MgAc2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
