3BD8

Glucogen Phosphorylase complex with 1(-D-glucopyranosyl) cytosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	CCD
Collection date	2005-02-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.8113
Spacegroup name	P 43 21 2
Unit cell lengths	128.591, 128.591, 116.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.490 - 2.100
R-factor	0.1803
Rwork	0.180
R-free	0.21080
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	57366
<I/σ(I)>	11.3	3.7
Completeness [%]	99.5	99.6
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10mM Bes buffer, 3mM DDT, pH6.7, SMALL TUBES, temperature 289K