3BCV
Crystal structure of a putative glycosyltransferase from Bacteroides fragilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 152.434, 152.434, 152.434 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.830 - 2.350 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.083 | 0.448 |
| Number of reflections | 23202 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 98.4 | 84.6 |
| Redundancy | 19.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Sodium acetate trihydrate, 0.2M Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
