3BCT

THE ARMADILLO REPEAT REGION FROM MURINE BETA-CATENIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	FUJI
Spacegroup name	C 2 2 21
Unit cell lengths	64.100, 102.000, 187.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.229
Rwork	0.229
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	16.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.069	0.407
Number of reflections	66300
<I/σ(I)>	7.5	1.5
Completeness [%]	99.4	94.5 *
Redundancy	2.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	10.5 *	4 *	0.5% (W/V) PEG 8000, 2.4 M UREA, 200 MM TRIS-HCL, PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	beta-59	7 (mg/ml)
2	1	drop	CAPS	10 (mM)
3	1	drop		50 (mM)
4	1	drop	DTT	1 (mM)
5	1	reservoir	PEG8000	0.5 (%(w/v))
6	1	reservoir	Tris-HCl	200 (mM)
7	1		urea	1.5-2.4 (M)