3BCP

Crystal Structure of The Swapped non covalent form of P19A/L28Q/N67D BS-RNase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	58.722, 86.404, 99.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.570
R-factor	0.282
Rwork	0.238
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r5d
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.810
High resolution limit [Å]	2.470
Rmerge	0.080
Number of reflections	17849
<I/σ(I)>	14
Completeness [%]	96.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	27% w/v PEG 8000, 0.1M sodium phosphate, 0.2M calcium chloride, 0.1M cacodilate pH 5.3-5.9, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K