3BC1
Crystal Structure of the complex Rab27a-Slp2a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.505, 77.777, 115.011 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.20145 |
| Rwork | 0.199 |
| R-free | 0.24474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2if0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.054 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.345 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.093 | 0.634 |
| Number of reflections | 45347 | |
| <I/σ(I)> | 6.5 | 1.25 |
| Completeness [%] | 95.7 | 98.6 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | Na Citrate, Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
