3BAV
Crystal structure of L26A/D28N mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-08-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.777, 96.950, 108.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.560 - 1.620 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.560 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.073 | 0.326 |
| Number of reflections | 44914 | |
| <I/σ(I)> | 44.62 | 3.34 |
| Completeness [%] | 91.3 | 85.4 |
| Redundancy | 7.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 3.3M Na-formate, 0.4M (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
